Journal of Hematology, ISSN 1927-1212 print, 1927-1220 online, Open Access
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Case Report

Volume 13, Number 3, June 2024, pages 99-103

A Hemoglobinopathy That Produces an Array of Different Hemoglobin A1c Values

Figures

Figure 1.
Figure 1. (a) Hemoglobin A1c analysis using BioRad D100 HPLC (A1c 11.5% NGSP) (102 mmol/mol IFCC). (b) Hemoglobin electrophoresis by CZE (Sebia Capillarys). This method does not show the HbA1c. CZE: capillary zone electrophoresis; IFCC: The International Federation of Clinical Chemistry and Laboratory Medicine; NGSP: National Glycohemoglobin Standardization Program.
Figure 2.
Figure 2. The specimen was analyzed by two additional separation modalities. (a) Tosoh G8 HPLC (A1c 4.4% NGSP (25 mmol/mol IFCC)). The P00, P-HV3 and P02 are variant peaks that would have prevented release of the A1c result. (b) Sebia Capillarys HbA1c Mode (A1c 5.7% NGSP (39 mmol/mol IFCC)). Refer to Table 1. HPLC: high-performance liquid chromatography; IFCC: The International Federation of Clinical Chemistry and Laboratory Medicine; NGSP: National Glycohemoglobin Standardization Program.

Table

Table 1. Hemoglobin A1c Determination by Four Different Methods
 
MethodA1c, % (NGSP)A1c, mmol/mol (IFCC)PrincipleFigure
*Unreportable because of unidentified peaks on the HPLC tracing. HPLC: high-performance liquid chromatography; IFCC: The International Federation of Clinical Chemistry and Laboratory Medicine; NGSP: National Glycohemoglobin Standardization Program.
BioRad D100 HPLC11.5102Cation exchange HPLC1a
Tosoh G8 HPLC4.4*25*Cation exchange HPLC2a
Sebia Capillarys A1c mode5.739Capillary zone electrophoresis, A1c mode2b
Beckman Coulter AU6805.233ImmunoassayNo applicable figure