Journal of Hematology, ISSN 1927-1212 print, 1927-1220 online, Open Access
Article copyright, the authors; Journal compilation copyright, J Hematol and Elmer Press Inc
Journal website http://www.thejh.org

Original Article

Volume 7, Number 1, January 2018, pages 7-13

Role of Nucleophosmin Gene Mutation in Leukemogenesis of Acute Myeloid Leukemia

Figures

Figure 1.
Figure 1. Images of DAPI mounted cytospins of colchicine treated HL60 cells, where blocked metaphase 1 is short, thick chromosomes, and blocked metaphase 2 is fused chromosomes. Nota bene (N.B.) similar results were obtained with OCI-AML3 cells.
Figure 2.
Figure 2. Single parameter histograms of the percentage of the blocked metaphases in colchicine treated OCI-AML3 and HL60 cells after 4 h, and the percentage of normal metaphases in untreated OCI-AML3 and HL60, after the same duration.
Figure 3.
Figure 3. (a) Immunofluorescence images of resting HL60 cells, immunostained with wtNPM (FC61991), with TR (red) conjugated secondary antibody, and nuclei were stained with DAPI (blue). N.B. similar images were obtained with colchicine treated and control cytospins. (b) Immunofluorescence images of actively dividing HL60 cells, immunostained with wtNPM (FC61991), with TR (red) conjugated secondary antibody, and nuclei were stained with DAPI (blue). N.B. similar images were obtained with colchicine treated and control cytospins.
Figure 4.
Figure 4. Immunofluorescence images of control, colchicine treated HL60 and OCI-AML3, as indicated in the figure, immunostained with antiNPM (ab 15440) with FITC (green) conjugated secondary antibody, and nuclei were stained with DAPI (blue).
Figure 5.
Figure 5. Immunoblotting results of wtNPM in lysates of (a) G0 and colch HL60, (b) G0 and colch OCI-AML3, results were scanned and edited with Epson scanner and program.
Figure 6.
Figure 6. Immunoblotting results of antiNPM (ab15440) in lysates of (a) G0 and colch HL60, (b) G0 and colch OCI-AML3, results were scanned and edited with Epson scanner and program.