Figure 1. Analysis of hemolysis by CHUHE assay. DHTR subject’s erythrocytes were incubated with her plasma in complement inhibitory buffer (C’ inhib) or complement permissive buffer (sera + RBC). Increasing concentrations of PA-dPEG24 (PIC1) were added to the complement permissive reaction. Data (n = 3) are means of independent experiments ± SEM. Five-pointed star denotes P = 0.059. DHTR: delayed hemolytic transfusion reaction; CHUHE: complement hemolysis using human erythrocytes; RBC: red blood cell; SEM: standard error of mean.