Platelet Activation in Stored Platelet Concentrates: Comparison of Two Methods Preparation

Abstract

Background: This study has determined in vitro quality of 5 days stored platelet concentrates prepared by two different methods. Preparation conditions of platelet may cause platelet activation, which contributes to decreased ability of stored platelet to function. The quality platelets concentrate plays an important role in transfusion therapy. Their quality was assessed using the following parameters: platelets, leukocytes and erythrocytes counts, pH, P-selcetin (CD62P) and Annexin V. P-selectin and Annexin V can be detected on the activated platelet. Annexin V was used as a parameter for quality monitoring of platelet concentrates during storage. The present paper compares quality properties of both platelet preparations in vitro.

Methods: In this experimental study, 30 platelet concentrates prepared with platelet rich plasma- platelet concentrates and 30 units via buffy coat-derived platelet concentrate methods. The percentages of Annexin V, P-selcetin expression, platelet, leukocytes and erythrocytes counts and pH were evaluated.

Results: During storage for up to 5 days, buffy coat-derived platelet concentrates units displayed, no significant pH, difference in comparison with platelet rich plasma- platelet concentrates preparation (P greater than 0.05). The mean leukocytes count buffy coat-derived platelet concentrates and platelet rich plasma- platelet concentrates was comparable and statistically significant difference was observed (P less than 0.05). During storage for up to 5days platelet rich plasma- platelet concentrates units displayed significant an increase in the CD62P, Annexin V expressions, as compared with buffy coat-derived platelet concentrates preparation on day 5 (P less than 0.05).

Conclusions: The kinetics of CD62P and annexin V levels are influenced by the method used to prepare platelets for storage. The different levels of CD62P and annexin V in buffy coat-derived platelet concentrates and platelet rich plasma- platelet concentrates clearly demonstrating a progressive activation process of platelet rich plasma- platelet concentrates exceeds that of buffy coat-derived platelet concentrates. 




doi:10.4021/jh103e